Oleocanthal-rich extra virgin olive oil demonstrates acute anti-platelet effects in healthy men in a randomized trial


  • EVOO varietal differences influence platelet aggregation and oxylipinproduction.
  • Inhibition of platelet aggregation correlated to oleocanthal content.
  • Inhibition of eicosanoids correlated to EVOO total phenolic content.
  • Basal diet may influence platelet inhibition responses to EVOOs.


The phenolic profiles of extra virgin olive oils (EVOOs) may influence their cardiovascular benefits. In a randomized crossover of acute EVOO intake on platelet function, participants (n = 9) consumed 40 mL of EVOO weekly. EVOOs were matched for total phenolic content and were either tyrosol-poor with 1:2 oleacein/oleocanthal (D2i0.5), or 2:1 oleacein/oleocanthal (D2i2), or predominantly tyrosol (D2i0). Ibuprofen provided a platelet inhibition control. Blood was collected pre- and 2 h post-EVOO intake. D2i0.5 and D2i2 reduced 1 µg/mL collagen-stimulated maximum platelet aggregation (Pmax), with effects best correlated to oleocanthal intake (R = 0.56, P = 0.002). Total phenolic intake was independently correlated to eicosanoid production inhibition, suggesting that cyclooxygenase blockade was not responsible for the Pmax inhibition. Five participants exhibited >25% ΔPmax declines with D2i0.5 and D2i2 intake and plasma metabolomic profiles discriminated subjects by oil responsivity. Platelet responses to acute EVOO intake are associated with oil phenolic composition and may be influenced by diet.

Published in: Journal of Functional Foods
Authors: KaranAgrawalabEleniMellioucXueqiLidTheresa L.Pedersene1Selina C.WangdfProkopiosMagiatiscJohn W.NewmanabeRoberta R.Holta
Source: ScienceDirect